Terminator
MaTif

Part:BBa_K2127007

Designed by: Mirat Sojitra   Group: iGEM16_IngenuityLab_Canada   (2016-10-14)


Terminator.png

biologyrrnBT1-T7TE
chassisE.coli
directionForward
forward_efficiencyUnknown
reverse_efficiencyUnknown

MT - TERMINATOR

  • Derivative of double terminator BBa_B0015
  • Nine base pairs removed from the forward 3' end to fit gBlock requirements
  • Further Characterization on activity needed


Secondary Structure of BBa_K2127007


Secondary Structure mFold was used to generate the secondary structure of the terminator. The part is derived from BBa_B0010 forming the long palindromic sequence and the BBa_B0012 sequence formimg the relative bottom hairpin. The last nine base pairs of the 3' have been removed compared to the BBa_B0015 part.




Measurement This BioBrick has not been measured. Below is a link to experimental protocols related to the characterization of terminator sequences.

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
[edit]
Categories
//terminator
Parameters
biologyrrnBT1-T7TE
chassisE.coli
directionForward
forward_efficiencyUnknown
reverse_efficiencyUnknown